Cloning of yeast gene for trichodermin resistance and ribosomal protein L3.

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Cloning of yeast gene for trichodermin resistance and ribosomal protein L3.

Yeast cells sensitive to the eukaryotic protein synthesis inhibitor trichodermin have been transformed with autonomously replicating recombinant plasmids carrying DNA fragments of the genome of a trichodermin-resistant yeast strain. After selection for trichodermin-resistant cells, several transformants yielded a plasmid containing a 13.5-kilobase (kb) DNA fragment that encodes the trichodermin...

متن کامل

Identification and characterization of the gene for Drosophila L3 ribosomal protein.

A cDNA clone that encodes a Drosophila homologue of ribosomal protein L3 was isolated from a Drosophila ovary gridded cDNA library. The Drosophila ribosomal protein L3 gene (RpL3) is highly conserved with ribosomal protein L3 genes in other organisms. It is a single copy gene and maps to position 86D5-10 on polytene chromosomes. A Minute gene in this region, M(3) 86D, is a possible candidate to...

متن کامل

Introduction of a Modified Ribosomal Protein L3 Gene as a Strategy to Increase Trichothecene Toxin Resistance in Plants

Fusarium graminearum, the causative agent of wheat head blight, produces the trichothecene deoxynivalenol (DON). DON acts as a protein synthesis inhibitor and is a significant factor contributing to the disease. However, acetylation of the C-3 hydroxyl group of a number of different trichothecenes reduces the toxicity to yeast as well as to the singlecelled plant, Chlamydomonas, suggesting that...

متن کامل

Mutations in ribosomal protein L3 are associated with oxazolidinone resistance in staphylococci of clinical origin.

Following recent reports of ribosomal protein L3 mutations in laboratory-derived linezolid-resistant (LZD(r)) Staphylococcus aureus, we investigated whether similar mutations were present in LZD(r) staphylococci of clinical origin. Sequence analysis of a variety of LZD(r) isolates revealed two L3 mutations, DeltaSer145 (S. aureus NRS127) and Ala157Arg (Staphylococcus epidermidis 1653059), both ...

متن کامل

Resistance to the peptidyl transferase inhibitor tiamulin caused by mutation of ribosomal protein l3.

The antibiotic tiamulin targets the 50S subunit of the bacterial ribosome and interacts at the peptidyl transferase center. Tiamulin-resistant Escherichia coli mutants were isolated in order to elucidate mechanisms of resistance to the drug. No mutations in the rRNA were selected as resistance determinants using a strain expressing only a plasmid-encoded rRNA operon. Selection in a strain with ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 1981

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.78.1.238